wizard dna isolation Search Results


genomic dna human blood  (Promega)
90
Promega genomic dna human blood
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Genomic Dna Human Blood, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna human blood/product/Promega
Average 90 stars, based on 1 article reviews
genomic dna human blood - by Bioz Stars, 2026-03
90/100 stars
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plasmid dna isolated using wizard megaprep columns  (Promega)
90
Promega plasmid dna isolated using wizard megaprep columns
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Plasmid Dna Isolated Using Wizard Megaprep Columns, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna isolated using wizard megaprep columns/product/Promega
Average 90 stars, based on 1 article reviews
plasmid dna isolated using wizard megaprep columns - by Bioz Stars, 2026-03
90/100 stars
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plasmidial dna isolated from bacterial cultures using wizard plus sv minipreps  (Promega)
90
Promega plasmidial dna isolated from bacterial cultures using wizard plus sv minipreps
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Plasmidial Dna Isolated From Bacterial Cultures Using Wizard Plus Sv Minipreps, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmidial dna isolated from bacterial cultures using wizard plus sv minipreps/product/Promega
Average 90 stars, based on 1 article reviews
plasmidial dna isolated from bacterial cultures using wizard plus sv minipreps - by Bioz Stars, 2026-03
90/100 stars
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wizard pcr preps dna purificationsystem (promega) gel isolation kit  (Promega)
90
Promega wizard pcr preps dna purificationsystem (promega) gel isolation kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Pcr Preps Dna Purificationsystem (Promega) Gel Isolation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard pcr preps dna purificationsystem (promega) gel isolation kit/product/Promega
Average 90 stars, based on 1 article reviews
wizard pcr preps dna purificationsystem (promega) gel isolation kit - by Bioz Stars, 2026-03
90/100 stars
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dna isolation kit for mammalian blood wizard genomic dna purification kit  (Promega)
90
Promega dna isolation kit for mammalian blood wizard genomic dna purification kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Dna Isolation Kit For Mammalian Blood Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna isolation kit for mammalian blood wizard genomic dna purification kit/product/Promega
Average 90 stars, based on 1 article reviews
dna isolation kit for mammalian blood wizard genomic dna purification kit - by Bioz Stars, 2026-03
90/100 stars
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plasmid dna isolation kit wizard plus sv minipreps  (Promega)
90
Promega plasmid dna isolation kit wizard plus sv minipreps
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Plasmid Dna Isolation Kit Wizard Plus Sv Minipreps, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna isolation kit wizard plus sv minipreps/product/Promega
Average 90 stars, based on 1 article reviews
plasmid dna isolation kit wizard plus sv minipreps - by Bioz Stars, 2026-03
90/100 stars
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double-stranded dna isolated from mutant colonies wizard minipreps  (Promega)
90
Promega double-stranded dna isolated from mutant colonies wizard minipreps
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Double Stranded Dna Isolated From Mutant Colonies Wizard Minipreps, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/double-stranded dna isolated from mutant colonies wizard minipreps/product/Promega
Average 90 stars, based on 1 article reviews
double-stranded dna isolated from mutant colonies wizard minipreps - by Bioz Stars, 2026-03
90/100 stars
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dna-isolation kits  (Gentra Systems)
90
Gentra Systems dna-isolation kits
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Dna Isolation Kits, supplied by Gentra Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna-isolation kits/product/Gentra Systems
Average 90 stars, based on 1 article reviews
dna-isolation kits - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
wizard miniprep for plasmid dna isolation  (Promega)
90
Promega wizard miniprep for plasmid dna isolation
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Miniprep For Plasmid Dna Isolation, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard miniprep for plasmid dna isolation/product/Promega
Average 90 stars, based on 1 article reviews
wizard miniprep for plasmid dna isolation - by Bioz Stars, 2026-03
90/100 stars
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plasmid dna extracted with a wizard plus sv miniprep spin kit  (Promega)
90
Promega plasmid dna extracted with a wizard plus sv miniprep spin kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Plasmid Dna Extracted With A Wizard Plus Sv Miniprep Spin Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna extracted with a wizard plus sv miniprep spin kit/product/Promega
Average 90 stars, based on 1 article reviews
plasmid dna extracted with a wizard plus sv miniprep spin kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
wizard magnetic 96 dna isolation kit  (Promega)
90
Promega wizard magnetic 96 dna isolation kit
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Wizard Magnetic 96 Dna Isolation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wizard magnetic 96 dna isolation kit/product/Promega
Average 90 stars, based on 1 article reviews
wizard magnetic 96 dna isolation kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
plasmid dna isolated by alkaline lysis/column purification techniques wizard plus minipreps  (Promega)
90
Promega plasmid dna isolated by alkaline lysis/column purification techniques wizard plus minipreps
Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell <t>genomic</t> <t>DNA.</t> C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.
Plasmid Dna Isolated By Alkaline Lysis/Column Purification Techniques Wizard Plus Minipreps, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid dna isolated by alkaline lysis/column purification techniques wizard plus minipreps/product/Promega
Average 90 stars, based on 1 article reviews
plasmid dna isolated by alkaline lysis/column purification techniques wizard plus minipreps - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Journal: BMC Genomics

Article Title: Human glycolipid transfer protein ( GLTP ) genes: organization, transcriptional status and evolution

doi: 10.1186/1471-2164-9-72

Figure Lengend Snippet: Methylation Status of Human Intronless GLTP Gene . Vertical bars show location of CpG dinucleotides. Bst UI restriction sites, consisting of adjacent CpG dinucleotides, are shown by scissors. Individual clones are shown by horizontal rows of circles. Filled circles represent methylated CpG dinucleotides; unfilled circles represent nonmethylated CpG dinucleotides. Primers for RT-PCR (255 base product) are indicated by dotted arrows. The ATG start codon represents nucleotides +1, +2, and +3. B. & C . Combined bisulfite restriction analysis (COBRA) of intronless GLTP gene methylation status. B . Agarose gel electrophoresis patterns obtained before (Lane 1) and after (Lane 2) Bst UI restriction digestion of bisulfite-treated PCR products for intronless GLTP gene ( GLTPi ) in human blood cell genomic DNA. C . COBRA analysis of intronless GLTP methylation status in different cell types. Lane 1 = pooled PCR products before Bst UI restriction digestion; Lanes 2–9 shows action of Bst UI restriction digestion. Lane 2 = glioma cells; Lane 3 = human skin fibroblasts; Lane 4 = human HBL100 breast cancer cells; Lane 5 = Gaucher cells; Lane 6 = human T47D breast cancer cells; Lane 7 = human IMR32 neuroblastoma cells; Lane 8 = human HTB126 breast cancer cells; Lane 9 = human Caov3 ovarian cancer cells. Because of the absence of Bst UI restriction sites in amplified PCR products of the CpG island of the 5-exon/4-intron region, COBRA analysis could be used to verify the absence of methylation detected by direct sequencing and served only as a negative control for Bst UI action shown Panel C. Std = molecular weight standards.

Article Snippet: Genomic DNA from human blood (Promega) or isolated from various cell lines (Sigma-Aldrich, GenElute™ mammalian genomic DNA miniprep kit) was bisulfite converted using manufacturer's recommendations (CpGenome Fast; Chemicon, Temecula, CA).

Techniques: Methylation, Clone Assay, Reverse Transcription Polymerase Chain Reaction, Combined Bisulfite Restriction Analysis Assay, Agarose Gel Electrophoresis, Amplification, Sequencing, Negative Control, Molecular Weight